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ProductsBack/FASN Antibody
Western Blot analysis of A549 cells using Fatty Acid Synthase Polyclonal Antibody
Western Blot analysis of A549 cells using Fatty Acid Synthase Polyclonal Antibody
Western Blot analysis of A549 cells using Fatty Acid Synthase Polyclonal Antibody

FASN Antibody

CSB-PA002507
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetFASN
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Overview

  • Supplier
    Cusabio
  • Product Name
    FASN Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2194
  • Target name
    FASN
  • Target description
    fatty acid synthase
  • Target synonyms
    FAS; fatty acid synthase; OA-519; SDR27X1; short chain dehydrogenase/reductase family 27X, member 1; type I fatty acid synthase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49327
  • Protein Name
    Fatty acid synthase
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FASN using anti-FASN antibody (PB9865). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 4: human 293T whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human RT4 whole cell lysates, Lane 7: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FASN antigen affinity purified polyclonal antibody (Catalog # PB9865) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FASN at approximately 273 kDa. The expected band size for FASN is at 273 kDa.
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GeneTex
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