Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brown adipocyte. Fatty Acid Synthase stained by Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX109833/GTX109833_40030_20210225_IHC-P_M_w_23060500_346.webp "Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brown adipocyte. Fatty Acid Synthase stained by Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX109833/GTX109833_40030_20141114_IHC_3_w_23060500_446.webp "Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min")
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX109833/GTX109833_44111_20201113_WB_w_23060500_982.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse white adipocyte. Fatty Acid Synthase stained by Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX109833/GTX109833_44111_20220114_IHC-P_M_1_w_23060500_649.webp "Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse white adipocyte. Fatty Acid Synthase stained by Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Fatty Acid Synthase protein stained by Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX109833/GTX109833_40030_20170913_IFA_w_23060500_244.webp "Fatty Acid Synthase antibody [N1], N-term detects Fatty Acid Synthase protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Fatty Acid Synthase protein stained by Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:500. Blue: Hoechst 33342 staining.")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX109833/GTX109833_40030_20160616_WB_shRNA_watermark_w_23060500_576.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [N1], N-term (GTX109833) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Fatty Acid Synthase antibody [N1], N-term
GTX109833
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFASN
Overview
- SupplierGeneTex
- Product NameFatty Acid Synthase antibody [N1], N-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.2 mg/ml
- ConjugateUnconjugated
- Gene ID2194
- Target nameFASN
- Target descriptionfatty acid synthase
- Target synonymsFAS, OA-519, SDR27X1, fatty acid synthase, 3-hydroxyacyl-[acyl-carrier-protein] dehydratase, 3-oxoacyl-[acyl-carrier-protein] reductase, 3-oxoacyl-[acyl-carrier-protein] synthase, Acyl-[acyl-carrier-protein] hydrolase, Enoyl-[acyl-carrier-protein] reductase, [Acyl-carrier-protein] S-acetyltransferase, [Acyl-carrier-protein] S-malonyltransferase, short chain dehydrogenase/reductase family 27X, member 1, type I fatty acid synthase
- HostRabbit
- IsotypeIgG
- Protein IDP49327
- Protein NameFatty acid synthase
- Scientific DescriptionThe enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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![Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Fatty Acid Synthase antibody [HL2160] (GTX638138) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638138/GTX638138_T-44928_20230210_WB_M_liver_23021401_510.webp)
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