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ProductsBack/Fatty Acid Synthase antibody [N1], N-term

Fatty Acid Synthase antibody [N1], N-term

GTX109833
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetFASN
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Overview

  • Supplier
    GeneTex
  • Product Name
    Fatty Acid Synthase antibody [N1], N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2194
  • Target name
    FASN
  • Target description
    fatty acid synthase
  • Target synonyms
    FAS; fatty acid synthase; OA-519; SDR27X1; short chain dehydrogenase/reductase family 27X, member 1; type I fatty acid synthase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49327
  • Protein Name
    Fatty acid synthase
  • Scientific Description
    The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Senescent Endothelial Cells Sustain Their Senescence-Associated Secretory Phenotype (SASP) through Enhanced Fatty Acid Oxidation.
    Read more
  • Therapeutic Effect of Curcumin on 5/6Nx Hypertriglyceridemia: Association with the Improvement of Renal Mitochondrial beta-Oxidation and Lipid Metabolism in Kidney and Liver.
    Read more
  • Dual-tracer positron emission tomography/computed tomography as an imaging probe of de novo lipogenesis in preclinical models of hepatocellular carcinoma.
    Read more
  • 3,5-Dicaffeoylquinic Acid Lowers 3T3-L1 Mitotic Clonal Expansion and Adipocyte Differentiation by Enhancing Heme Oxygenase-1 Expression. Raineri A et al., 2021 Aug 19, Molecules
    Read more
  • Loss of Acot12 contributes to NAFLD independent of lipolysis of adipose tissue. Park S et al., 2021 Jul, Exp Mol Med
    Read more
  • Antrodia cinnamomea Confers Obesity Resistance and Restores Intestinal Barrier Integrity in Leptin-deficient Obese Mice. Tsai YT et al., 2020 Mar 10, Nutrients
    Read more
  • Allopurinol Prevents the Lipogenic Response Induced by an Acute Oral Fructose Challenge in Short-Term Fructose Fed Rats. Garcia-Arroyo FE et al., 2019 Oct 11, Biomolecules
    Read more
  • Clinical importance of FASN in relation to HIF-1alpha and SREBP-1c in gastric adenocarcinoma. Ezzeddini R et al., 2019 May 1, Life Sci
    Read more
  • Fenofibrate induces human hepatoma Hep3B cells apoptosis and necroptosis through inhibition of thioesterase domain of fatty acid synthase. You BJ et al., 2019 Mar 1, Sci Rep
    Read more
  • Dietary supplementation with myo-inositol reduces hepatic triglyceride accumulation and expression of both fructolytic and lipogenic genes in rats fed a high-fructose diet. Shimada M et al., 2017 Nov, Nutr Res
    Read more

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Figure 1. Western blot analysis of FASN using anti-FASN antibody (PB9865). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 4: human 293T whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human RT4 whole cell lysates, Lane 7: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FASN antigen affinity purified polyclonal antibody (Catalog # PB9865) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FASN at approximately 273 kDa. The expected band size for FASN is at 273 kDa.
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