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ProductsBack/FBXO32 / Fbx32 Antibody

FBXO32 / Fbx32 Antibody

LS-C830020
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetFBXO32
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    FBXO32 / Fbx32 Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:5000 - 1:10000), IHC (1:30 - 1:150)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID114907
  • Target name
    FBXO32
  • Target description
    F-box protein 32
  • Target synonyms
    Fbx32, MAFbx, F-box only protein 32, atrogin 1, muscle atrophy F-box protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Fbx32/FBXO32 using anti-Fbx32/FBXO32 antibody (A02531-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse heart tissue lysates, Lane 2: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fbx32/FBXO32 antigen affinity purified polyclonal antibody (Catalog # A02531-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fbx32/FBXO32 at approximately 42 kDa. The expected band size for Fbx32/FBXO32 is at 42 kDa.
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