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Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: FDPS antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 49, 41 kDa Observed band size: 41 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: FDPS antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 49, 41 kDa Observed band size: 41 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: FDPS antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 49, 41 kDa Observed band size: 41 kDa

FDPS Antibody

CSB-PA008563LA01HU
Cusabio
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFDPS
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Overview

  • Supplier
    Cusabio
  • Product Name
    FDPS Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2224
  • Target name
    FDPS
  • Target description
    farnesyl diphosphate synthase
  • Target synonyms
    FPPS, FPS, POROK9, farnesyl pyrophosphate synthase, (2E,6E)-farnesyl diphosphate synthase, FPP synthase, FPP synthetase, farnesyl pyrophosphate synthetase, dimethylallyltranstransferase, geranyltranstransferase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14324
  • Protein Name
    Farnesyl pyrophosphate synthase
  • Scientific Description
    Key enzyme in isoprenoid biosynthesis which catalyzes the formation of farnesyl diphosphate (FPP), a precursor for several classes of essential metabolites including sterols, dolichols, carotenoids, and ubiquinones. FPP also serves as substrate for protein farnesylation and geranylgeranylation. Catalyzes the sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphates, dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate to the ultimate product farnesyl pyrophosphate.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of FDPS using anti-FDPS antibody (A01782-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FDPS antigen affinity purified polyclonal antibody (Catalog # A01782-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FDPS at approximately 40KD. The expected band size for FDPS is at 40KD.
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