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ProductsBack/FDX1 / ADX Antibody

FDX1 / ADX Antibody

LS-C411342
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetFDX1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    FDX1 / ADX Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 19kDa, while the observed MW by Western blot was 14kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), WB (1:200 - 1:500) The predicted MW is 19kDa, while the observed MW by Western blot was 14kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2230
  • Target name
    FDX1
  • Target description
    ferredoxin 1
  • Target synonyms
    ADX, FDX, LOH11CR1D, adrenodoxin, mitochondrial, adrenal ferredoxin, hepatoredoxin, mitochondrial adrenodoxin
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FDX1 using anti-FDX1 antibody (M05441). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: human SiHa whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FDX1 antigen affinity purified monoclonal antibody (Catalog # M05441) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FDX1 at approximately 13 kDa. The expected band size for FDX1 is at 19 kDa.
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