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Western Blot analysis of HuvEc cells using FEN-1 Polyclonal Antibody
Western Blot analysis of HuvEc cells using FEN-1 Polyclonal Antibody
Western Blot analysis of HuvEc cells using FEN-1 Polyclonal Antibody

FEN1 Antibody

CSB-PA002510
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFEN1
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Overview

  • Supplier
    Cusabio
  • Product Name
    FEN1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2237
  • Target name
    FEN1
  • Target description
    flap structure-specific endonuclease 1
  • Target synonyms
    FEN-1, MF1, RAD2, flap endonuclease 1, DNase IV, maturation factor-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP39748
  • Protein Name
    Flap endonuclease 1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FEN1 using anti-FEN1 antibody (A01484-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Daudi whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FEN1 antigen affinity purified polyclonal antibody (Catalog # A01484-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FEN1 at approximately 43 kDa. The expected band size for FEN1 is at 43 kDa.
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