Western Blot Positive WB detected in: Hela whole cell lysate, Raji whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate All lanes: FEN1 antibody at 0.775microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 43, 36 KDa Observed band size: 43 KDa
Western Blot Positive WB detected in: Hela whole cell lysate, Raji whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate All lanes: FEN1 antibody at 0.775microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 43, 36 KDa Observed band size: 43 KDa
FEN1 Recombinant Monoclonal Antibody
CSB-RA008585A0HU
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFEN1
Overview
- SupplierCusabio
- Product NameFEN1 Recombinant Monoclonal Antibody
- Delivery Days Customer20
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID4D9
- ConjugateUnconjugated
- Gene ID2237
- Target nameFEN1
- Target descriptionflap structure-specific endonuclease 1
- Target synonymsDNase IV; FEN-1; flap endonuclease 1; maturation factor-1; MF1; RAD2
- IsotypeIgG
- Protein IDP39748
- Protein NameFlap endonuclease 1
- Scientific DescriptionStructure-specific nuclease with 5-flap endonuclease and 5-3 exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5-end of a downstream Okazaki fragment. It enters the flap from the 5-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5-3 exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
- ReactivityHuman
- Storage Instruction-20°C or -80°C
- UNSPSC12352203