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The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA049381(FHIT Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA049381(FHIT Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA049381(FHIT Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: x200)

FHIT Antibody

CSB-PA049381
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFHIT
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Overview

  • Supplier
    Cusabio
  • Product Name
    FHIT Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2272
  • Target name
    FHIT
  • Target description
    fragile histidine triad diadenosine triphosphatase
  • Target synonyms
    AP3Aase, FRA3B, bis(5'-adenosyl)-triphosphatase, AP3A hydrolase, adenosine 5'-monophosphoramidase FHIT, adenylylsulfatase, adenylylsulfate-ammonia adenylyltransferase, diadenosine 5',5'''-P1,P3-triphosphate hydrolase, dinucleosidetriphosphatase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49789
  • Protein Name
    Bis(5'-adenosyl)-triphosphatase
  • Scientific Description
    This gene, a member of the histidine triad gene family, encodes a diadenosine 5,5-P1,P3-triphosphate hydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Alternatively spliced transcript variants have been found for this gene.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FHIT using anti-FHIT antibody (PB9181). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FHIT antigen affinity purified polyclonal antibody (Catalog # PB9181) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FHIT at approximately 17 kDa. The expected band size for FHIT is at 17 kDa.
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