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Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA617906LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA617906LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA617906LA01HU at dilution of 1:100

FHL2 Antibody

CSB-PA617906LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetFHL2
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Overview

  • Supplier
    Cusabio
  • Product Name
    FHL2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2274
  • Target name
    FHL2
  • Target description
    four and a half LIM domains 2
  • Target synonyms
    AAG11; aging-associated gene 11; down-regulated in rhabdomyosarcoma LIM protein; DRAL; FHL-2; four and a half LIM domains protein 2; LIM domain protein DRAL; skeletal muscle LIM-protein 3; SLIM3; SLIM-3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14192
  • Protein Name
    Four and a half LIM domains protein 2
  • Scientific Description
    May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FHL2 using anti-FHL2 antibody (A02129-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FHL2 antigen affinity purified polyclonal antibody (Catalog # A02129-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FHL2 at approximately 32 kDa. The expected band size for FHL2 is at 32 kDa.
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