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Goat anti-FHL2

EB06303
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetFHL2
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-FHL2 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID2274
  • Target name
    FHL2
  • Target description
    four and a half LIM domains 2
  • Target synonyms
    AAG11; aging-associated gene 11; down-regulated in rhabdomyosarcoma LIM protein; DRAL; FHL-2; four and a half LIM domains protein 2; LIM domain protein DRAL; skeletal muscle LIM-protein 3; SLIM3; SLIM-3
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001441.4. GeneIDs all Nonhuman: 14200 (mouse); 63839 (rat);. Purification: Antigen affinity purified. Names and symbols: FHL2; DRAL; AAG11; SLIM3; four and a half LIM domains 2; down-regulated in rhabdomyosarcoma LIM protein; aging-associated gene 11; four and a half LIM-domain protein 2; OTTHUMP00000203164; OTTHUMP00000203168; OTTHUMP00000203169
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FHL2 using anti-FHL2 antibody (A02129-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FHL2 antigen affinity purified polyclonal antibody (Catalog # A02129-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FHL2 at approximately 32 kDa. The expected band size for FHL2 is at 32 kDa.
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