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Fibrinogen beta antibody [C1C3]

GTX113694
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetFGB
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Overview

  • Supplier
    GeneTex
  • Product Name
    Fibrinogen beta antibody [C1C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:5000-1:50000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.94 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2244
  • Target name
    FGB
  • Target description
    fibrinogen beta chain
  • Target synonyms
    HEL-S-78p, fibrinogen beta chain, beta-fibrinogen, epididymis secretory sperm binding protein Li 78p, fibrinogen, B beta polypeptide
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP02675
  • Protein Name
    Fibrinogen beta chain
  • Scientific Description
    The protein encoded by this gene is the beta component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including afibrinogenemia, dysfibrinogenemia, hypodysfibrinogenemia and thrombotic tendency. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Chuang YC, Lei HY, Lin YS, et al. Dengue virus-induced autoantibodies bind to plasminogen and enhance its activation. J Immunol. 2011,187(12):6483-90. doi: 10.4049/jimmunol.1102218
    Read this paper

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Figure 1. Western blot analysis of Fibrinogen beta chain/FGB using anti-Fibrinogen beta chain/FGB antibody (A01204-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat kidney tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Fibrinogen beta chain/FGB antigen affinity purified polyclonal antibody (Catalog # A01204-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Fibrinogen beta chain/FGB at approximately 56 kDa. The expected band size for Fibrinogen beta chain/FGB is at 56 kDa.
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