Fibronectin antibody [N1N2], N-term detects Fibronectin protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. Fibronectin stained by Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
![Fibronectin antibody [N1N2], N-term detects Fibronectin protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. Fibronectin stained by Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX112794/GTX112794_44601_20220617_IHC-P_M_22062919_713.webp "Fibronectin antibody [N1N2], N-term detects Fibronectin protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. Fibronectin stained by Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112794/GTX112794_44510_20211126_WB_treatment_Nicotinamide_TSA_w_23060500_969.webp "Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Fibronectin antibody [N1N2] detects Fibronectin protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2] (GTX112794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112794/GTX112794_41633_20151015_WB_M_plasma_w_23060500_886.webp "Fibronectin antibody [N1N2] detects Fibronectin protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Fibronectin antibody [N1N2] (GTX112794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Indirect ELISA analysis was performed by coating plate with 100 μL of recombinant Fibronectin protein at concentration of 10 μg/mL. The coated protein is detected with Fibronectin antibody [N1N2], N-term (GTX112794) at rangeing from 0.5 to 140 ng/mL.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112794/GTX112794_41941_20180608_ELISA_w_23060500_472.webp "Indirect ELISA analysis was performed by coating plate with 100 μL of recombinant Fibronectin protein at concentration of 10 μg/mL. The coated protein is detected with Fibronectin antibody [N1N2], N-term (GTX112794) at rangeing from 0.5 to 140 ng/mL.")
![Fibronectin antibody [N1N2], N-term detects FN1 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. Fibronectin antibody [N1N2], N-term (GTX112794) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX112794/GTX112794_40079_IHC_w_23060500_555.webp "Fibronectin antibody [N1N2], N-term detects FN1 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. Fibronectin antibody [N1N2], N-term (GTX112794) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min")
![Fibronectin antibody [N1N2], N-term immunoprecipitates Fibronectin protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of Fibronectin protein expressing HeLa cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of Fibronectin by 3 μg of Fibronectin antibody [N1N2], N-term (GTX112794) 5% SDS-PAGE The immunoprecipitated Fibronectin protein was detected by Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX112794/GTX112794_40079_IP_w_23060500_732.webp "Fibronectin antibody [N1N2], N-term immunoprecipitates Fibronectin protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of Fibronectin protein expressing HeLa cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of Fibronectin by 3 μg of Fibronectin antibody [N1N2], N-term (GTX112794) 5% SDS-PAGE The immunoprecipitated Fibronectin protein was detected by Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.")
Fibronectin antibody [N1N2], N-term detects Fibronectin protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. Fibronectin stained by Fibronectin antibody [N1N2], N-term (GTX112794) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Fibronectin antibody [N1N2], N-term
GTX112794
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFN1
Overview
- SupplierGeneTex
- Product NameFibronectin antibody [N1N2], N-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. ELISA: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.48 mg/ml
- ConjugateUnconjugated
- Gene ID2335
- Target nameFN1
- Target descriptionfibronectin 1
- Target synonymsCIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, SMDCF, fibronectin, cold-insoluble globulin, epididymis secretory sperm binding protein, lnc-ABCA12-8, migration-stimulating factor
- HostRabbit
- IsotypeIgG
- Protein IDP02751
- Protein NameFibronectin
- Scientific DescriptionThis gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Ragni M, Canciani L, Spataro L, et al. An amino acid mixture, enriched with Krebs cycle intermediates, enhances extracellular matrix gene expression in cultured human fibroblasts. Amino Acids. 2023,55(11):1679-1685. doi: 10.1007/s00726-023-03340-yRead this paper
- Hung TH, Huang Y, Yeh CT, et al. High expression of embryonic stem cell marker SSEA3 confers poor prognosis and promotes epithelial mesenchymal transition in hepatocellular carcinoma. Biomed J. 2024,47(2):100612. doi: 10.1016/j.bj.2023.100612Read this paper
- Li YJ, Shi JR, Li SC, et al. Phosphodiesterase type 10A inhibitor attenuates lung fibrosis by targeting myofibroblast activation. iScience. 2023,26(5):106586. doi: 10.1016/j.isci.2023.106586Read this paper
- Chiou WC, Huang GJ, Chang TY, et al. Ovatodiolide inhibits SARS-CoV-2 replication and ameliorates pulmonary fibrosis through suppression of the TGF-β/TβRs signaling pathway. Biomed Pharmacother. 2023,161:114481. doi: 10.1016/j.biopha.2023.114481Read this paper
- Chen H, Ma X, Zhang M, et al. Injectable and biofunctionalized fibrin hydrogels co-embedded with stem cells induce hair follicle genesis. Regen Biomater. 2023,10:rbac086. doi: 10.1093/rb/rbac086Read this paper
- Su MT, Tsai CW, Tsai PY, et al. Aspirin Inhibits Fibronectin Expression and Reverses Fibronectin-Mediated Cell Invasiveness by Activating Akt Signaling in Preeclampsia. Pharmaceuticals (Basel). 2022,15(12). doi: 10.3390/ph15121523Read this paper
- Chua HH, Chang MH, Chen YH, et al. PIM1-Induced Cytoplasmic Expression of RBMY Mediates Hepatocellular Carcinoma Metastasis. Cell Mol Gastroenterol Hepatol. 2023,15(1):121-152. doi: 10.1016/j.jcmgh.2022.09.014Read this paper
- Zhao W, Chen H, Zhang Y, et al. Adaptive multi-degree-of-freedom in situ bioprinting robot for hair-follicle-inclusive skin repair: A preliminary study conducted in mice. Bioeng Transl Med. 2022,7(3):e10303. doi: 10.1002/btm2.10303Read this paper
- Catarinella G, Nicoletti C, Bracaglia A, et al. SerpinE1 drives a cell-autonomous pathogenic signaling in Hutchinson-Gilford progeria syndrome. Cell Death Dis. 2022,13(8):737. doi: 10.1038/s41419-022-05168-yRead this paper
- Lee WJ, Cheng TC, Yen Y, et al. Tea polyphenol epigallocatechin-3-gallate inhibits cell proliferation in a patient-derived triple-negative breast cancer xenograft mouse model via inhibition of proline-dehydrogenase-induced effects. J Food Drug Anal. 2021,29(1):113-127. doi: 10.38212/2224-6614.3230Read this paper
Datasheet
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![IHC-P analysis of human pancreatic adenocarcinoma tissue using GTX34725 Fibronectin antibody [HFN7.1].](https://www.genetex.com/upload/website/prouct_img/normal/GTX34725/GTX34725_20200115_IHC-P_908_w_23060801_383.webp)
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