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Mouse anti Human Fibronectin

X1263M
Nordic-MUbio
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetFN1
100 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Human Fibronectin
  • Delivery Days Customer
    7
  • Application Supplier Note
    Antibody can be used for staining of human and mouse frozen and paraffin-embedded tissue sections. Optimal concentration should be evaluated by serial dilutions.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Immunohistochemistry (frozen & paraffin)
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    EP5
  • Conjugate
    Unconjugated
  • Gene ID2335
  • Target name
    FN1
  • Target description
    fibronectin 1
  • Target synonyms
    CIG; cold-insoluble globulin; ED-B; epididymis secretory sperm binding protein; fibronectin; FINC; FN; FNZ; GFND; GFND2; LETS; migration-stimulating factor; MSF; SMDCF
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP02751
  • Protein Name
    Fibronectin
  • Scientific Description
    Fibronectin
  • Shelf life instruction
    See expiration date on vial
  • Source
    Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with T-cell lymphoma biopsy and mouse myeloma cells.
  • Reactivity Supplier
    Human;Mouse
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FN1 using anti-FN1 antibody (A00564-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 3: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FN1 antigen affinity purified polyclonal antibody (Catalog # A00564-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FN1 at approximately 220-400 kDa. The expected band size for FN1 is at 272 kDa.
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