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ProductsBack/FKBP12 (FKBP1A) Mouse Monoclonal Antibody [Clone ID: OTI3B3]
Figure A, Western blot analysis of overexpressed lysates (25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human FKBP1A plasmid (RC201237, hFKBP1A), mouse FKBP1A plasmid (MR200405, mFKBP1A) using anti-FKBP1A antibody TA500557 (1:5000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:5000@1mg/ml).
Figure A, Western blot analysis of overexpressed lysates (25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human FKBP1A plasmid (RC201237, hFKBP1A), mouse FKBP1A plasmid (MR200405, mFKBP1A) using anti-FKBP1A antibody TA500557 (1:5000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:5000@1mg/ml).
Figure A, Western blot analysis of overexpressed lysates (25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human FKBP1A plasmid (RC201237, hFKBP1A), mouse FKBP1A plasmid (MR200405, mFKBP1A) using anti-FKBP1A antibody TA500557 (1:5000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:5000@1mg/ml).

FKBP12 (FKBP1A) Mouse Monoclonal Antibody [Clone ID: OTI3B3]

CF500557
OriGene
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFKBP1A
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    FKBP12 (FKBP1A) Mouse Monoclonal Antibody [Clone ID: OTI3B3]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI3B3
  • Gene ID2280
  • Target name
    FKBP1A
  • Target description
    FKBP prolyl isomerase 1A
  • Target synonyms
    FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12, PKCI2, PPIASE, peptidyl-prolyl cis-trans isomerase FKBP1A, 12 kDa FK506-binding protein, 12 kDa FKBP, FK506 binding protein 1A, 12kDa, FK506 binding protein12, FK506-binding protein 1, FK506-binding protein 12, FK506-binding protein 1A, FK506-binding protein, T-cell, 12-kD, FKBP12-Exip3, PPIase FKBP1A, calstabin-1, immunophilin FKBP12, protein kinase C inhibitor 2, rotamase
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP62942
  • Protein Name
    Peptidyl-prolyl cis-trans isomerase FKBP1A
  • Scientific Description
    Carrier-free (BSA/glycerol-free) FKBP1A mouse monoclonal antibody, clone OTI3B3 (formerly 3B3)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FKBP1A/1B using anti-FKBP1A/1B antibody (A04492-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: huamn MOLT-4 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FKBP1A/1B antigen affinity purified polyclonal antibody (Catalog # A04492-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FKBP1A/1B at approximately 15 kDa. The expected band size for FKBP1A/1B is at 12 kDa.
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