More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/FLAP antibody, Internal
WB analysis of U937 cell lysate using GTX89246 FLAP antibody, Internal. Dilution : 0.1microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of U937 cell lysate using GTX89246 FLAP antibody, Internal. Dilution : 0.1microg/ml Loading : 35microg protein in RIPA buffer
WB analysis of U937 cell lysate using GTX89246 FLAP antibody, Internal. Dilution : 0.1microg/ml Loading : 35microg protein in RIPA buffer

FLAP antibody, Internal

GTX89246
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetALOX5AP
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    FLAP antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.1-0.3microg/ml. IHC-P: 3-6microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.50 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID241
  • Target name
    ALOX5AP
  • Target description
    arachidonate 5-lipoxygenase activating protein
  • Target synonyms
    arachidonate 5-lipoxygenase-activating protein; FLAP; MK-886-binding protein
  • Host
    Goat
  • Isotype
    IgG
  • Protein IDP20292
  • Protein Name
    Arachidonate 5-lipoxygenase-activating protein
  • Scientific Description
    This gene encodes a protein which, with 5-lipoxygenase, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
References
Bioss Antibodies
ALOX5AP Polyclonal AntibodyBS-7556R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetALOX5AP
  • SizePrice
Product group Antibodies
MedChem Express
FLAP Antibody (YA2703, Rabbit)HY-P82958
TargetALOX5AP
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ALOX5AP / FLAP AntibodyLS-C807165
ApplicationsWestern Blot, ELISA
TargetALOX5AP
  • SizePrice
Immunohistochemistry analysis in human bone marrow and skeletal muscle tissues using Anti-ALOX5AP antibody. Corresponding ALOX5AP RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-ALOX5AP AntibodyHPA026592
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetALOX5AP
  • SizePrice
Product group Antibodies
Nordic-MUbio
Rabbit anti Human FLAPX2318P
ApplicationsWestern Blot
TargetALOX5AP
  • SizePrice
Figure 1. Western blot analysis of ALOX5AP using anti-ALOX5AP antibody (A02341). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALOX5AP antigen affinity purified polyclonal antibody (Catalog # A02341) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALOX5AP at approximately 18 kDa. The expected band size for ALOX5AP is at 18 kDa.
Product group Antibodies
Boster Bio
Anti-ALOX5AP Antibody Picoband(r)A02341-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetALOX5AP
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-FLAP / ALOX5AP (C Term)EB06394
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry
TargetALOX5AP
  • SizePrice
IHC-P analysis of human lung using GTX89725 FLAP antibody, C-term. Antigen retrieval : citrate buffer pH 6 Dilution : 3.75microg/ml
Product group Antibodies
GeneTex
FLAP antibody, C-termGTX89725
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetALOX5AP
  • SizePrice