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FLAP Antibody (YA2703, Rabbit)

HY-P82958
MedChem Express
TargetALOX5AP
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    FLAP Antibody (YA2703, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID241
  • Target name
    ALOX5AP
  • Target description
    arachidonate 5-lipoxygenase activating protein
  • Target synonyms
    arachidonate 5-lipoxygenase-activating protein; FLAP; MK-886-binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    FLAP Antibody (YA2703) is a non-conjugated IgG antibody, targeting FLAP, with a predicted molecular weight of 18 kDa (observed band size: 18 kDa). FLAP Antibody (YA2703) can be used for WB experiment in human background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ALOX5AP using anti-ALOX5AP antibody (A02341). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALOX5AP antigen affinity purified polyclonal antibody (Catalog # A02341) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALOX5AP at approximately 18 kDa. The expected band size for ALOX5AP is at 18 kDa.
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