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Hela lysates probed with FLNB Polyclonal Antibody, Unconjugated (bs-13181R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Hela lysates probed with FLNB Polyclonal Antibody, Unconjugated (bs-13181R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Hela lysates probed with FLNB Polyclonal Antibody, Unconjugated (bs-13181R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

FLNB Polyclonal Antibody

BS-13181R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetFLNB
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    FLNB/Filamin 3 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2317
  • Target name
    FLNB
  • Target description
    filamin B
  • Target synonyms
    ABP-278; ABP-280; ABP-280 homolog; actin binding protein 278; actin-binding-like protein; AOI; beta-filamin; FH1; filamin B, beta; filamin homolog 1; filamin-3; filamin-B; FLN1L; FLN-B; Larsen syndrome 1 (autosomal dominant); LRS1; SCT; TABP; TAP; thyroid autoantigen
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Filamin B/FLNB using anti-Filamin B/FLNB antibody (A02562-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human U20S whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse Ana-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Filamin B/FLNB antigen affinity purified polyclonal antibody (Catalog # A02562-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Filamin B/FLNB at approximately 278 kDa. The expected band size for Filamin B/FLNB is at 278 kDa.
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References
GeneTex
Filamin B antibody [N1], N-termGTX101206
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TargetFLNB
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