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FLT4 / VEGFR3 Antibody

LS-C832540
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
TargetFLT4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    FLT4 / VEGFR3 Antibody
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, ELISA
  • Applications Supplier
    ELISA (1:5000 - 1:60000), IF (1:100 - 1:500)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.08 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2324
  • Target name
    FLT4
  • Target description
    fms related receptor tyrosine kinase 4
  • Target synonyms
    CHTD7; Feline McDonough Sarcoma (FMS)-like tyrosine kinase 4; FLT-4; FLT41; fms related tyrosine kinase 4; fms-like tyrosine kinase 4; LMPH1A; LMPHM1; PCL; primary congenital lymphedema; tyrosine-protein kinase receptor FLT4; vascular endothelial growth factor receptor 3; VEGF receptor-3; VEGFR3; VEGFR-3
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of VEGF Receptor 3 using anti-VEGF Receptor 3 antibody (A01276-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF Receptor 3 antigen affinity purified polyclonal antibody (Catalog # A01276-2) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGF Receptor 3 at approximately 153KD. The expected band size for VEGF Receptor 3 is at 153KD.
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