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ProductsBack/FluoSite(tm) Anti-Nectin4 Antibody, PE-Labeled

FluoSite(tm) Anti-Nectin4 Antibody, PE-Labeled

102637
BPS Bioscience
ApplicationsFlow Cytometry
Product group Antibodies
TargetNECTIN4
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Overview

  • Supplier
    BPS Bioscience
  • Product Name
    FluoSite(tm) Anti-Nectin4 Antibody, PE-Labeled
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    RPE
  • Estimated Purity
    ≥90%
  • Gene ID81607
  • Target name
    NECTIN4
  • Target description
    nectin cell adhesion molecule 4
  • Target synonyms
    EDSS1; Ig superfamily receptor LNIR; LNIR; nectin 4; nectin-4; poliovirus receptor-related 4; poliovirus receptor-related protein 4; PRR4; PVRL4
  • Isotype
    IgG1
  • Protein IDQ96NY8
  • Protein Name
    Nectin-4
  • Scientific Description
    Anti-Nectin4 is a purified monospecific recombinant antibody, which is labeled with R-Phycoerythrin (PE). This antibody has been tested by flow cytometry for binding to Human Nectin4 on the surface of Nectin4 CHO Cell Line (High) (BPS Bioscience #78097-H). FluoSite™ is a tag-based method for site-specific conjugation of antibodies with fluorophores, ensuring the labeling site is far from the antigen-binding domain to preserve antibody functionality. This precise, consistent labeling ensures the homogeneity of the labeled antibody preparation, making it ideal for flow cytometry and other high-sensitivity applications.
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Nectin-4/PVRL4 using anti-Nectin-4/PVRL4 antibody (PB10091). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: MCF-7 whole cell lysates, Lane 2: MM453 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nectin-4/PVRL4 antigen affinity purified polyclonal antibody (Catalog # PB10091) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nectin-4/PVRL4 at approximately 66 kDa. The expected band size for Nectin-4/PVRL4 is at 55 kDa.
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