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Nectin4 Polyclonal Antibody

CAC11459
Biomatik
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
TargetNECTIN4
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Overview

  • Supplier
    Biomatik
  • Product Name
    Nectin4 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, ELISA
  • Applications Supplier
    ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID81607
  • Target name
    NECTIN4
  • Target description
    nectin cell adhesion molecule 4
  • Target synonyms
    EDSS1, LNIR, PRR4, PVRL4, nectin-4, nectin-4, Ig superfamily receptor LNIR, nectin 4, poliovirus receptor-related 4, poliovirus receptor-related protein 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96NY8
  • Protein Name
    Nectin-4
  • Scientific Description
    The Nectin4 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, IF.
  • Reactivity Supplier
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Nectin-4/PVRL4 using anti-Nectin-4/PVRL4 antibody (PB10091). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: MCF-7 whole cell lysates, Lane 2: MM453 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nectin-4/PVRL4 antigen affinity purified polyclonal antibody (Catalog # PB10091) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Nectin-4/PVRL4 at approximately 66 kDa. The expected band size for Nectin-4/PVRL4 is at 55 kDa.
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