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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA008746ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA008746ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA008746ESR2HU at dilution of 1:100

FMO1 Antibody

CSB-PA008746ESR2HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetFMO1
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Overview

  • Supplier
    Cusabio
  • Product Name
    FMO1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2326
  • Target name
    FMO1
  • Target description
    flavin containing dimethylaniline monoxygenase 1
  • Target synonyms
    dimethylaniline monooxygenase [N-oxide-forming] 1; dimethylaniline oxidase 1; fetal hepatic flavin-containing monooxygenase 1; flavin containing monooxygenase 1; Flavin-containing monooxygenase 1 (fetal liver); FMO 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01740
  • Protein Name
    Dimethylaniline monooxygenase [N-oxide-forming] 1
  • Scientific Description
    This protein is involved in the oxidative metabolism of a variety of xenobiotics such as drugs and pesticides. Form I catalyzes the N-oxygenation of secondary and tertiary amines.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FMO1 using anti-FMO1 antibody (PB9589). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: Mouse Liver Tissue Lysate at 50ug, Lane 3: Rat Kidney Tissue Lysate at 50ug, Lane 4: Mouse Kidney Tissue Lysate at 50ug, Lane 5: SMMC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FMO1 antigen affinity purified polyclonal antibody (Catalog # PB9589) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FMO1 at approximately 60 kDa. The expected band size for FMO1 is at 60 kDa.
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