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Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA857449ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA857449ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA857449ESR1HU at dilution of 1:100

FMO2 Antibody

CSB-PA857449ESR1HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFMO2
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Overview

  • Supplier
    Cusabio
  • Product Name
    FMO2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2327
  • Target name
    FMO2
  • Target description
    flavin containing dimethylaniline monoxygenase 2
  • Target synonyms
    FMO1B1, flavin-containing monooxygenase 2, FMO, pulmonary, dimethylaniline monooxygenase [N-oxide-forming] 2, dimethylaniline oxidase 2, flavin containing monooxygenase 2 (non-functional), pulmonary flavin-containing monooxygenase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99518
  • Protein Name
    Flavin-containing monooxygenase 2
  • Scientific Description
    Catalyzes the N-oxidation of certain primary alkylamines to their oximes via an N-hydroxylamine intermediate. Inactive toward certain tertiary amines, such as imipramine or chloropromazine. Can catalyze the S-oxidation of methimazole. The truncated form is catalytically inactive.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FMO2 using anti-FMO2 antibody (PB9760). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: A549 Whole Cell Lysate, Lane 2: HELA Whole Cell Lysate, Lane 3: MCF-7 Whole Cell Lysate, Lane 4: SW620 Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FMO2 antigen affinity purified polyclonal antibody (Catalog # PB9760) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FMO2 at approximately 54 kDa. The expected band size for FMO2 is at 54 kDa.
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