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FMO2 Antibody

LS-C748996
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFMO2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    FMO2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 53kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 53kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.66 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2327
  • Target name
    FMO2
  • Target description
    flavin containing dimethylaniline monoxygenase 2
  • Target synonyms
    FMO1B1, flavin-containing monooxygenase 2, FMO, pulmonary, dimethylaniline monooxygenase [N-oxide-forming] 2, dimethylaniline oxidase 2, flavin containing monooxygenase 2 (non-functional), pulmonary flavin-containing monooxygenase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FMO2 using anti-FMO2 antibody (PB9760). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: A549 Whole Cell Lysate, Lane 2: HELA Whole Cell Lysate, Lane 3: MCF-7 Whole Cell Lysate, Lane 4: SW620 Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FMO2 antigen affinity purified polyclonal antibody (Catalog # PB9760) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FMO2 at approximately 54 kDa. The expected band size for FMO2 is at 54 kDa.
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