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Western Blot analysis of HeLa cells using FMO3 Polyclonal Antibody.
Western Blot analysis of HeLa cells using FMO3 Polyclonal Antibody.
Western Blot analysis of HeLa cells using FMO3 Polyclonal Antibody.

FMO3 Antibody

CSB-PA005810
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetFMO3
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Overview

  • Supplier
    Cusabio
  • Product Name
    FMO3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2328
  • Target name
    FMO3
  • Target description
    flavin containing dimethylaniline monoxygenase 3
  • Target synonyms
    dimethylaniline monooxygenase [N-oxide-forming] 3; dimethylaniline oxidase 3; dJ127D3.1; flavin containing monooxygenase 3; FMO form 2; FMOII; hepatic flavin-containing monooxygenase-3; TMAU; trimethylamine monooxygenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP31513
  • Protein Name
    Dimethylaniline monooxygenase [N-oxide-forming] 3
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FMO3 using anti-FMO3 antibody (PB9590). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: Mouse Liver Tissue Lysate at 50ug, Lane 3: SMMC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FMO3 antigen affinity purified polyclonal antibody (Catalog # PB9590) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FMO3 at approximately 60 kDa. The expected band size for FMO3 is at 60 kDa.
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