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FMR1 / FMRP Antibody (aa164-200)

LS-C407915
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetFMR1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    FMR1 / FMRP Antibody (aa164-200)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (0.1 - 0.5 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2332
  • Target name
    FMR1
  • Target description
    fragile X messenger ribonucleoprotein 1
  • Target synonyms
    FMRP, FRAXA, POF, POF1, fragile X messenger ribonucleoprotein 1, FMRP translational regulator 1, synaptic functional regulator FMR1
  • Host
    Rabbit
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FMRP/FMR1 using anti-FMRP/FMR1 antibody (PB9703). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FMRP/FMR1 antigen affinity purified polyclonal antibody (Catalog # PB9703) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FMRP/FMR1 at approximately 71-73 kDa. The expected band size for FMRP/FMR1 is at 71 kDa.
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