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Gel: 8%SDS-PAGE,Lysate: 40ug,Primary antibody: CSB-PA167678(FOXA2 Antibody) at dilution 1/500 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 3 minutes
Gel: 8%SDS-PAGE,Lysate: 40ug,Primary antibody: CSB-PA167678(FOXA2 Antibody) at dilution 1/500 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 3 minutes
Gel: 8%SDS-PAGE,Lysate: 40ug,Primary antibody: CSB-PA167678(FOXA2 Antibody) at dilution 1/500 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 3 minutes

FOXA2 Antibody

CSB-PA167678
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFOXA2
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Overview

  • Supplier
    Cusabio
  • Product Name
    FOXA2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3170
  • Target name
    FOXA2
  • Target description
    forkhead box A2
  • Target synonyms
    HNF-3-beta, HNF3B, TCF3B, hepatocyte nuclear factor 3-beta, forkhead box protein A2, hepatic nuclear factor-3-beta, transcription factor 3B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y261
  • Protein Name
    Hepatocyte nuclear factor 3-beta
  • Scientific Description
    This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific genes such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. This gene has been linked to sporadic cases of maturity-onset diabetes of the young. Transcript variants encoding different isoforms have been identified for this gene.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of FOXA2 using anti-FOXA2 antibody (A01032-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: rat pancreas tissue lysates, Lane 7: mouse pancreas tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXA2 antigen affinity purified polyclonal antibody (Catalog # A01032-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXA2 at approximately 48KD. The expected band size for FOXA2 is at 48KD.
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