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HNF3beta/FOXA2 Monoclonal Antibody

CAB19053
Assay Genie
TargetFOXA2
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    HNF3bèta/FOXA2 Rabbit mAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IF,IP
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID3170
  • Target name
    FOXA2
  • Target description
    forkhead box A2
  • Target synonyms
    forkhead box protein A2; hepatic nuclear factor-3-beta; hepatocyte nuclear factor 3-beta; HNF3B; HNF-3-beta; TCF3B; transcription factor 3B
  • Host
    Rabbit
  • Protein IDQ9Y261
  • Protein Name
    Hepatocyte nuclear factor 3-beta
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FOXA2 using anti-FOXA2 antibody (A01032-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: rat pancreas tissue lysates, Lane 7: mouse pancreas tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXA2 antigen affinity purified polyclonal antibody (Catalog # A01032-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXA2 at approximately 48KD. The expected band size for FOXA2 is at 48KD.
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