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ProductsBack/FOXA2 Mouse Monoclonal Antibody [Clone ID: 7H4B7]
Western blot analysis using FOXA2 mouse mAb against A549 (1) cell lysate.
Western blot analysis using FOXA2 mouse mAb against A549 (1) cell lysate.
Western blot analysis using FOXA2 mouse mAb against A549 (1) cell lysate.

FOXA2 Mouse Monoclonal Antibody [Clone ID: 7H4B7]

AM06359SU-N
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetFOXA2
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    FOXA2 Mouse Monoclonal Antibody [Clone ID: 7H4B7]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    7H4B7
  • Gene ID3170
  • Target name
    FOXA2
  • Target description
    forkhead box A2
  • Target synonyms
    HNF-3-beta, HNF3B, TCF3B, hepatocyte nuclear factor 3-beta, forkhead box protein A2, hepatic nuclear factor-3-beta, transcription factor 3B
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9Y261
  • Protein Name
    Hepatocyte nuclear factor 3-beta
  • Scientific Description
    FOXA2 mouse monoclonal antibody, clone 7H4B7, Ascites
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of FOXA2 using anti-FOXA2 antibody (A01032-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human Caco-2 whole cell lysates, Lane 6: rat pancreas tissue lysates, Lane 7: mouse pancreas tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXA2 antigen affinity purified polyclonal antibody (Catalog # A01032-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXA2 at approximately 48KD. The expected band size for FOXA2 is at 48KD.
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