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Foxi1 Polyclonal Antibody

CAC08199
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetFOXI1
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Overview

  • Supplier
    Biomatik
  • Product Name
    FOXI1 Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    ELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2299
  • Target name
    FOXI1
  • Target description
    forkhead box I1
  • Target synonyms
    FKH10; FKHL10; forkhead box protein I1; forkhead-like 10; forkhead-related activator 6; forkhead-related protein FKHL10; forkhead-related transcription factor 6; FREAC6; FREAC-6; hepatocyte nuclear factor 3 forkhead homolog 3; HFH3; HFH-3; HNF-3/fork-head homolog-3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12951
  • Protein Name
    Forkhead box protein I1
  • Scientific Description
    The Foxi1 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IF.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FOXI1 using anti-FOXI1 antibody (A07512-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates, Lane 2: monkey COS-7 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXI1 antigen affinity purified polyclonal antibody (Catalog # A07512-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXI1 at approximately 60KD. The expected band size for FOXI1 is at 41KD.
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