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Western Blot analysis of LOVO cells using FoxJ1 Polyclonal Antibody
Western Blot analysis of LOVO cells using FoxJ1 Polyclonal Antibody
Western Blot analysis of LOVO cells using FoxJ1 Polyclonal Antibody

FOXJ1 Antibody

CSB-PA008305
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFOXJ1
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Overview

  • Supplier
    Cusabio
  • Product Name
    FOXJ1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2302
  • Target name
    FOXJ1
  • Target description
    forkhead box J1
  • Target synonyms
    CILD43, FKHL13, HFH-4, HFH4, forkhead box protein J1, fork head homologue 4, forkhead transcription factor HFH-4, forkhead-like 13, forkhead-related protein FKHL13, hepatocyte nuclear factor 3 forkhead homolog 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92949
  • Protein Name
    Forkhead box protein J1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FOXJ1 using anti-FOXJ1 antibody (A04942-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW579 whole cell lysates, Lane 2: human SK-OV-3 whole cell lysates, Lane 3: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXJ1 antigen affinity purified polyclonal antibody (Catalog # A04942-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXJ1 at approximately 55 kDa. The expected band size for FOXJ1 is at 55 kDa.
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