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Anti-FOXJ1 Antibody

101-10562
RayBiotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetFOXJ1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-FOXJ1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID2302
  • Target name
    FOXJ1
  • Target description
    forkhead box J1
  • Target synonyms
    CILD43, FKHL13, HFH-4, HFH4, forkhead box protein J1, fork head homologue 4, forkhead transcription factor HFH-4, forkhead-like 13, forkhead-related protein FKHL13, hepatocyte nuclear factor 3 forkhead homolog 4
  • Host
    Mouse
  • Isotype
    IgG2a
  • Scientific Description
    Human FOXJ1 monoclonal antibody (100 ug)
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FOXJ1 using anti-FOXJ1 antibody (A04942-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW579 whole cell lysates, Lane 2: human SK-OV-3 whole cell lysates, Lane 3: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXJ1 antigen affinity purified polyclonal antibody (Catalog # A04942-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXJ1 at approximately 55 kDa. The expected band size for FOXJ1 is at 55 kDa.
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