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Western Blot analysis of Mouse brain and heart tissue using FOXL2 Polyclonal Antibody at dilution of 1:750
Western Blot analysis of Mouse brain and heart tissue using FOXL2 Polyclonal Antibody at dilution of 1:750
Western Blot analysis of Mouse brain and heart tissue using FOXL2 Polyclonal Antibody at dilution of 1:750

FOXL2 Polyclonal Antibody

E-AB-15650
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    FOXL2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB
  • Certification
    Research Use Only
  • Concentration
    0.5mg/ml
  • Scientific Description
    This gene encodes a forkhead transcription factor. The protein contains a fork-head DNA-binding domain and may play a role in ovarian development and function. Mutations in this gene are a cause of blepharophimosis syndrome and premature ovarian failure 3. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis through transcriptional repression of the Sertoli cell-promoting gene SOX9. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FOXL2 using anti-FOXL2 antibody (A01185-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat ovary tissue lysates, Lane 3: mouse ovary tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXL2 antigen affinity purified polyclonal antibody (Catalog # A01185-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXL2 at approximately 50 kDa. The expected band size for FOXL2 is at 50 kDa.
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