More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/FoxP3 Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with FoxP3 Polyclonal Antibody, Unconjugated (bs-10211R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with FoxP3 Polyclonal Antibody, Unconjugated (bs-10211R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat brain tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with FoxP3 Polyclonal Antibody, Unconjugated (bs-10211R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining

FoxP3 Polyclonal Antibody

bs-10211R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    FoxP3 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Doxorubicin-polyglycerol-nanodiamond conjugate is a cytostatic agent that evades chemoresistance and reverses cancer-induced immunosuppression in triple-negative breast cancer. Yuan SJ et al., 2019 Oct 17, J Nanobiotechnology
    Read more
  • Dendritic cell-mediated delivery of doxorubicin-polyglycerol-nanodiamond composites elicits enhanced anti-cancer immune response in glioblastoma. Li TF et al., 2018 Oct, Biomaterials
    Read more

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
RayBiotech
Anti-Human FoxP3 Antibody, Purified136-00050
ApplicationsFlow Cytometry
TargetFOXP3
  • SizePrice
FACS analysis of PFA-fixed Jurkat cells using GTX04725 FOXP3 antibody. Blue : Primary antibody Black : Isotype control Permeabilization : 0.5% Triton Dilution : 10 microg/mL
Product group Antibodies
GeneTex
FOXP3 antibodyGTX04725
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetFOXP3
  • SizePrice
Product group Antibodies
Nordic-MUbio
Rabbit anti Human Forkhead box protein P3X2347P
ApplicationsWestern Blot
TargetFOXP3
  • SizePrice
Figure 1. Western blot analysis of FOXP3 using anti-FOXP3 antibody (A00011-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A375 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXP3 antigen affinity purified polyclonal antibody (Catalog # A00011-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXP3 at approximately 47 kDa. The expected band size for FOXP3 is at 47 kDa.
Product group Antibodies
Boster Bio
Anti-FOXP3 Antibody Picoband(r)A00011-3-CARRIER-FREE
ApplicationsWestern Blot, ELISA
TargetFOXP3
  • SizePrice
Product group Antibodies
Biomatik
Foxp3 Polyclonal AntibodyCAC07030
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetFOXP3
  • SizePrice
Product group Antibodies
Atlas Antibodies
Anti-FOXP3 AntibodyAMAB92051
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetFOXP3
  • SizePrice
Anti-FOXP3 Antibody
Product group Antibodies
Antibodies.com
Anti-FOXP3 AntibodyA285957
ApplicationsELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-FOXP3 Recombinant Monoclonal Antibody [BLR034F]A700-034
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetFOXP3
  • SizePrice
Western Blot analysis of PC12 cells using FOXP3 Polyclonal Antibody.
Product group Antibodies
Cusabio
FOXP3 AntibodyCSB-PA005784
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetFOXP3
  • SizePrice
Product group Antibodies
MedChem Express
FOXP3 Antibody (YA759, Mouse)HY-P80678
TargetFOXP3
  • SizePrice