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Goat anti-FOXP3 / SCURFIN

EB05294
Everest Biotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetFOXP3
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-FOXP3 / SCURFIN Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the nucleoplasm of U2OS cells. Recommended concentration: 10microg/ml. Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml. Immunocytochemistry: CD25-sorted (Treg) Human blood cells with nuclear speckled staining. Recommended concentration 2-4ug/ml for overnight staining.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IF, FC, ICC, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID50943
  • Target name
    FOXP3
  • Target description
    forkhead box P3
  • Target synonyms
    AIID; DIETER; forkhead box protein P3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; IPEX; JM2; PIDX; XPID
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_054728.2; NP_001107849.1. Purification: Antigen affinity purified. Names and symbols: FOXP3; SCURFIN; forkhead box P3; JM2; AIID; IPEX; PIDX; XPID; DIETER; JM2 protein; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; MGC141961; MGC141963; FOXP3delta7; scurfin
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FOXP3 using anti-FOXP3 antibody (A00011-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A375 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXP3 antigen affinity purified polyclonal antibody (Catalog # A00011-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXP3 at approximately 47 kDa. The expected band size for FOXP3 is at 47 kDa.
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