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ProductsBack/FRA1 (FOSL1) Mouse Monoclonal Antibody [Clone ID: OTI12F9]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY FOSL1 (Cat# RC202104, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOSL1 (Cat#TA500624).
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY FOSL1 (Cat# RC202104, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOSL1 (Cat#TA500624).
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY FOSL1 (Cat# RC202104, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FOSL1 (Cat#TA500624).

FRA1 (FOSL1) Mouse Monoclonal Antibody [Clone ID: OTI12F9]

CF500624
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetFOSL1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    FRA1 (FOSL1) Mouse Monoclonal Antibody [Clone ID: OTI12F9]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI12F9
  • Gene ID8061
  • Target name
    FOSL1
  • Target description
    FOS like 1, AP-1 transcription factor subunit
  • Target synonyms
    FRA, FRA1, fra-1, fos-related antigen 1, FOS like 1, AP-1 trancription factor subunit, FOS-like antigen-1
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP15407
  • Protein Name
    Fos-related antigen 1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) FOSL1 mouse monoclonal antibody, clone OTI12F9 (formerly 12F9)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of FRA1 using anti-FRA1 antibody (A03927-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FRA1 antigen affinity purified polyclonal antibody (Catalog # A03927-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FRA1 at approximately 29KD. The expected band size for FRA1 is at 29KD.
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