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NIH-3T3 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with FRA2 antibody (GTX134241) diluted at 1:5000 and competitor's antibody (sc-604) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
NIH-3T3 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with FRA2 antibody (GTX134241) diluted at 1:5000 and competitor's antibody (sc-604) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.
NIH-3T3 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with FRA2 antibody (GTX134241) diluted at 1:5000 and competitor's antibody (sc-604) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. *The competitor is not affiliated with GeneTex and does not endorse this product.

FRA2 antibody

GTX134241
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetFOSL2
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Overview

  • Supplier
    GeneTex
  • Product Name
    FRA2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.66 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2355
  • Target name
    FOSL2
  • Target description
    FOS like 2, AP-1 transcription factor subunit
  • Target synonyms
    ACED, FRA2, fos-related antigen 2, FOS like 2, AP-1 trancription factor subunit, FOS like antigen 2, FRA-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15408
  • Protein Name
    Fos-related antigen 2
  • Scientific Description
    The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. [provided by RefSeq, Jul 2014]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FRA2 using anti-FRA2 antibody (PB9591). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Lung Tissue Lysate at 50ug, Lane 2: HEPG2 Whole Cell Lysate at 40ug, Lane 3: A549 Whole Cell Lysate at 40ug, Lane 4: SW620 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FRA2 antigen affinity purified polyclonal antibody (Catalog # PB9591) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FRA2 at approximately 43 kDa. The expected band size for FRA2 is at 35 kDa.
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