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FRA2 Recombinant Antibody

BSM-62266R
Bioss
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot
Product group Antibodies
TargetFOSL2
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Overview

  • Supplier
    Bioss
  • Product Name
    FRA2 Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IP(1-2ug)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID2355
  • Target name
    FOSL2
  • Target description
    FOS like 2, AP-1 transcription factor subunit
  • Target synonyms
    ACED, FRA2, fos-related antigen 2, FOS like 2, AP-1 trancription factor subunit, FOS like antigen 2, FRA-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP15408
  • Protein Name
    Fos-related antigen 2
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FRA2 using anti-FRA2 antibody (PB9591). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Lung Tissue Lysate at 50ug, Lane 2: HEPG2 Whole Cell Lysate at 40ug, Lane 3: A549 Whole Cell Lysate at 40ug, Lane 4: SW620 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FRA2 antigen affinity purified polyclonal antibody (Catalog # PB9591) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FRA2 at approximately 43 kDa. The expected band size for FRA2 is at 35 kDa.
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