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WB analysis of HepG2 lysate (30ug) using PFKM antibody [AT2F11] at the indicated dilutions.
WB analysis of HepG2 lysate (30ug) using PFKM antibody [AT2F11] at the indicated dilutions.
WB analysis of HepG2 lysate (30ug) using PFKM antibody [AT2F11] at the indicated dilutions.

PFKM antibody [AT2F11]

GTX53761
GeneTex
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetPFKM
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Overview

  • Supplier
    GeneTex
  • Product Name
    Fructose 6 Phosphate Kinase antibody [AT2F11]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:2000. Recommended starting dilution is 1:2000.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AT2F11
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5213
  • Target name
    PFKM
  • Target description
    phosphofructokinase, muscle
  • Target synonyms
    6-phosphofructo-1-kinase; 6-phosphofructokinase type A; 6-phosphofructokinase, muscle type; ATP-dependent 6-phosphofructokinase, muscle type; ATP-PFK; GSD7; PFK1; PFK-1; PFKA; PFK-A; PFKX; phosphofructo-1-kinase isozyme A; phosphofructokinase 1; phosphofructokinase, polypeptide X; phosphofructokinase-M; phosphohexokinase; PPP1R122; protein phosphatase 1, regulatory subunit 122
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP08237
  • Protein Name
    ATP-dependent 6-phosphofructokinase, muscle type
  • Scientific Description
    Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PFKM using anti-PFKM antibody (A00437-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PFKM antigen affinity purified polyclonal antibody (Catalog # A00437-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PFKM at approximately 85 kDa. The expected band size for PFKM is at 85 kDa.
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