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ProductsBack/FTHFSDC1 / MTHFD1L Antibody

FTHFSDC1 / MTHFD1L Antibody

LS-C409514
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetMTHFD1L
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    FTHFSDC1 / MTHFD1L Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 29kDa/105kDa, while the observed MW by Western blot was 106kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IF (1:50 - 1:100), IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 29kDa/105kDa, while the observed MW by Western blot was 106kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID25902
  • Target name
    MTHFD1L
  • Target description
    methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like
  • Target synonyms
    10-formyl-THF synthetase; dJ292B18.2; formyltetrahydrofolate synthetase domain containing 1; FTHFSDC1; monofunctional C1-tetrahydrofolate synthase, mitochondrial; MTC1THFS
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MTHFD1L using anti-MTHFD1L antibody (A06289-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTHFD1L antigen affinity purified polyclonal antibody (Catalog # A06289-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTHFD1L at approximately 106 kDa. The expected band size for MTHFD1L is at 106 kDa.
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