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Western blot All lanes: MTHFD1L antibody at 3.68microg/ml + Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 106, 30 kDa Observed band size: 106 kDa
Western blot All lanes: MTHFD1L antibody at 3.68microg/ml + Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 106, 30 kDa Observed band size: 106 kDa
Western blot All lanes: MTHFD1L antibody at 3.68microg/ml + Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 106, 30 kDa Observed band size: 106 kDa

MTHFD1L Antibody

CSB-PA754385ESR2HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMTHFD1L
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Overview

  • Supplier
    Cusabio
  • Product Name
    MTHFD1L Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID25902
  • Target name
    MTHFD1L
  • Target description
    methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like
  • Target synonyms
    FTHFSDC1, MTC1THFS, dJ292B18.2, monofunctional C1-tetrahydrofolate synthase, mitochondrial, 10-formyl-THF synthetase, formyltetrahydrofolate synthetase domain containing 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6UB35
  • Protein Name
    Monofunctional C1-tetrahydrofolate synthase, mitochondrial
  • Scientific Description
    May provide the missing metabolic reaction required to link the mitochondria and the cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic an transformed cells complementing thus the enzymatic activities of MTHFD2.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of MTHFD1L using anti-MTHFD1L antibody (A06289-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTHFD1L antigen affinity purified polyclonal antibody (Catalog # A06289-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MTHFD1L at approximately 106 kDa. The expected band size for MTHFD1L is at 106 kDa.
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