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ProductsBack/FAH antibody
Sample (30 ug of whole cell lysate) A: Hep G2 (GTX27900) 10% SDS PAGE GTX102583 diluted at 1:1000
Sample (30 ug of whole cell lysate) A: Hep G2 (GTX27900) 10% SDS PAGE GTX102583 diluted at 1:1000
Sample (30 ug of whole cell lysate) A: Hep G2 (GTX27900) 10% SDS PAGE GTX102583 diluted at 1:1000

FAH antibody

GTX102583
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetFAH
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Overview

  • Supplier
    GeneTex
  • Product Name
    FAH antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.13 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2184
  • Target name
    FAH
  • Target description
    fumarylacetoacetate hydrolase
  • Target synonyms
    fumarylacetoacetase, FAA, beta-diketonase, epididymis secretory sperm binding protein, fumarylacetoacetate hydrolase (fumarylacetoacetase)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP16930
  • Protein Name
    Fumarylacetoacetase
  • Scientific Description
    This gene encodes the last enzyme in the tyrosine catabolism pathway. FAH deficiency is associated with Type 1 hereditary tyrosinemia (HT). [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FAA/FAH using anti-FAA/FAH antibody (A02072-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCP tissue lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAA/FAH antigen affinity purified polyclonal antibody (Catalog # A02072-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAA/FAH at approximately 41 kDa. The expected band size for FAA/FAH is at 46 kDa.
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