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Western blot analysis of extracts from COLO205 cells, using FXR2 antibody.
Western blot analysis of extracts from COLO205 cells, using FXR2 antibody.
Western blot analysis of extracts from COLO205 cells, using FXR2 antibody.

FXR2 Antibody

CSB-PA870516
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetFXR2
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Overview

  • Supplier
    Cusabio
  • Product Name
    FXR2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9513
  • Target name
    FXR2
  • Target description
    FMR1 autosomal homolog 2
  • Target synonyms
    FMR1L2, FXR2P, RNA-binding protein FXR2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51116
  • Protein Name
    Fragile X mental retardation syndrome-related protein 2
  • Scientific Description
    RNA-binding protein. Wiley S.R., Immunity 3:673-682(1995). Pitti R.M., J. Biol. Chem. 271:12687-12690(1996). Hymowitz S.G., Mol. Cell 4:563-571(1999).
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of FXR2 using anti-FXR2 antibody (A05130-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: huamn U251 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FXR2 antigen affinity purified polyclonal antibody (Catalog # A05130-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FXR2 at approximately 93 kDa. The expected band size for FXR2 is at 74 kDa.
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