Fyn antibody

Biorbyt

Fyn antibody

16

Immunohistochemistry (paraffin sections): Recommended dilution: 5 microg/ml; positive tissue: brain. Western blotting: Recommended dilution: 2 microg/ml; positive control: JURKAT human T cell leukemia cell line. Immunoprecipitation: positive control: J77 T cell line. Preparation of cell lysate: 30 min on ice (orbital incubator) in lysing buffer, lysing buffer with N-dodecyl beta-D-maltoside (20 mM Tris/Cl, 100 mM NaCl pH 8,2, 1% laurylmaltosid (w/v), 50 mM NaF). The antibody FYN-01 apparently gives very specific signal (by 59 kDa), and nicely immunoprecipitates Fyn from cell lysates.. Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml; positive tissue: brain. Western blotting: Recommended dilution: 2 μg/ml; positive control: JURKAT human T cell leukemia cell line. Immunoprecipitation: positive control: J77 T cell line. Preparation of cell lysate: 30 min on ice (orbital incubator) in lysing buffer, lysing buffer with N-dodecyl beta-D-maltoside (20 mM Tris/Cl, 100 mM NaCl pH 8,2, 1% laurylmaltosid (w/v), 50 mM NaF). The antibody FYN-01 apparently gives very specific signal (by 59 kDa), and nicely immunoprecipitates Fyn from cell lysates. ICC, IHC-P, IP, WB

ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

FYN-01

1 mg/ml

Unconjugated

FYN

FYN proto-oncogene, Src family tyrosine kinase

c-syn protooncogene; FYN oncogene related to SRC, FGR, YES; OKT3-induced calcium influx regulator; p59-FYN; proto-oncogene c-Fyn; proto-oncogene Syn; SLK; src/yes-related novel; src-like kinase; SYN; tyrosine kinase p59fyn(T); tyrosine-protein kinase Fyn

IgG2b

Tyrosine-protein kinase Fyn

Mouse Monoclonal to Fyn.

Human, Mouse

-20°C,2°C to 8°C

12352203