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G3BP1 / G3BP Antibody

LS-C749818
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetG3BP1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    G3BP1 / G3BP Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 13kDa/52kDa, while the observed MW by Western blot was 68kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 13kDa/52kDa, while the observed MW by Western blot was 68kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10146
  • Target name
    G3BP1
  • Target description
    G3BP stress granule assembly factor 1
  • Target synonyms
    ATP-dependent DNA helicase VIII; DNA helicase VIII; G3BP; G3BP-1; GAP binding protein; GAP SH3 domain-binding protein 1; GTPase activating protein (SH3 domain) binding protein 1; HDH-VIII; ras GTPase-activating protein-binding protein 1; RasGAP-associated endoribonuclease G3BP; Ras-GTPase-activating protein SH3-domain-binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of G3BP/G3BP1 using anti-G3BP/G3BP1 antibody (A02199-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MDA-MB-453 whole cell lysates, Lane 5: human U-87MG whole cell lysates, Lane 6: human Caco-2 whole cell lysates, Lane 7: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-G3BP/G3BP1 antigen affinity purified polyclonal antibody (Catalog # A02199-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for G3BP/G3BP1 at approximately 68 kDa. The expected band size for G3BP/G3BP1 is at 68 kDa.
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