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ProductsBack/GAA / Alpha-Glucosidase, Acid Antibody (aa851-952, clone 3C6)

GAA / Alpha-Glucosidase, Acid Antibody (aa851-952, clone 3C6)

LS-C197260
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetGAA
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GAA / Alpha-Glucosidase, Acid Antibody (aa851-952, clone 3C6)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Sandwich ELISA: Recombinant protein. Western Blot: Recombinant protein.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB Sandwich ELISA: Recombinant protein. Western Blot: Recombinant protein.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3C6
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2548
  • Target name
    GAA
  • Target description
    alpha glucosidase
  • Target synonyms
    IOPD, LOPD, LYAG, lysosomal alpha-glucosidase, acid maltase, aglucosidase alfa, glucosidase alpha, acid
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GAA using anti-GAA antibody (A01548). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human PC-3 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAA antigen affinity purified polyclonal antibody (Catalog # A01548) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAA at approximately 110,95,76,70KD. The expected band size for GAA is at 110,95,76,70KD.
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