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ProductsBack/GAA Rabbit Polyclonal Antibody
WB Suggested Anti-GAA Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: MCF7 cell lysate;GAA is supported by BioGPS gene expression data to be expressed in MCF7
WB Suggested Anti-GAA Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: MCF7 cell lysate;GAA is supported by BioGPS gene expression data to be expressed in MCF7
WB Suggested Anti-GAA Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: MCF7 cell lysate;GAA is supported by BioGPS gene expression data to be expressed in MCF7

GAA Rabbit Polyclonal Antibody

TA334653
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGAA
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    GAA Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID2548
  • Target name
    GAA
  • Target description
    alpha glucosidase
  • Target synonyms
    IOPD, LOPD, LYAG, lysosomal alpha-glucosidase, acid maltase, aglucosidase alfa, glucosidase alpha, acid
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10253
  • Protein Name
    Lysosomal alpha-glucosidase
  • Scientific Description
    Rabbit Polyclonal Anti-GAA Antibody
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of GAA using anti-GAA antibody (A01548). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human PC-3 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAA antigen affinity purified polyclonal antibody (Catalog # A01548) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAA at approximately 110,95,76,70KD. The expected band size for GAA is at 110,95,76,70KD.
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