More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/GAA Rabbit Polyclonal Antibody
Western blot analysis of various lysates using GAA Rabbit pAb (TA376424) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.
Western blot analysis of various lysates using GAA Rabbit pAb (TA376424) at 1:1000 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25μg per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit .<br/>Exposure time: 60s.
Western blot analysis of various lysates using GAA Rabbit pAb (TA376424) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.

GAA Rabbit Polyclonal Antibody

TA376424
OriGene
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGAA
Price on request
100 ul
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    OriGene
  • Product Name
    GAA Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID2548
  • Target name
    GAA
  • Target description
    alpha glucosidase
  • Target synonyms
    IOPD, LOPD, LYAG, lysosomal alpha-glucosidase, acid maltase, aglucosidase alfa, glucosidase alpha, acid
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10253
  • Protein Name
    Lysosomal alpha-glucosidase
  • Scientific Description
    GAA Rabbit polyclonal Antibody
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

Download MSDS

FAQ

Go to our frequently asked questions

Related products

Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA009125LA01HU at dilution of 1:100
Product group Antibodies
Cusabio
GAA AntibodyCSB-PA009125LA01HU
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetGAA
  • SizePrice
Product group Antibodies
Assay Genie
GAA AntibodyCAB7674
ReactivityHuman
TargetGAA
  • SizePrice
Figure 1. Western blot analysis of GAA using anti-GAA antibody (A01548). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human PC-3 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAA antigen affinity purified polyclonal antibody (Catalog # A01548) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAA at approximately 110,95,76,70KD. The expected band size for GAA is at 110,95,76,70KD.
Product group Antibodies
Boster Bio
Anti-GAA Antibody Picoband(r)A01548-CARRIER-FREE
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetGAA
  • SizePrice
Anti-GAA Antibody
Product group Antibodies
Antibodies.com
Anti-GAA AntibodyA15834
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
MedChem Express
GAA Antibody (YA2872, Rabbit)HY-P83127
TargetGAA
  • SizePrice
Immunohistochemical staining of human epididymis shows strong granular cytoplasmic positivity in glandular cells.
Product group Antibodies
Atlas Antibodies
Anti-GAA AntibodyHPA026970
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetGAA
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
GAA / Alpha-Glucosidase, Acid Antibody (aa601-952)LS-C682186
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetGAA
  • SizePrice
Product group Antibodies
Nordic-MUbio
Mouse anti alpha-GlucosidaseMUB0707P
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetGAA
  • SizePrice
Product group Antibodies
Biomatik
Gaa Polyclonal AntibodyCAC07609
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
TargetGAA
  • SizePrice
Wild-type (WT) and LYAG knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LYAG antibody [C2C3], C-term (GTX109821) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
LYAG antibody [C2C3], C-termGTX109821
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetGAA
  • SizePrice