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GABARAP Antibody

LS-C813559
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetGABARAP
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GABARAP Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000 - 1:20000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID11337
  • Target name
    GABARAP
  • Target description
    GABA type A receptor-associated protein
  • Target synonyms
    ATG8A; epididymis secretory sperm binding protein; GABA(A) receptor-associated protein; GABARAP-a; gamma-aminobutyric acid receptor-associated protein; MM46
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GABARAP using anti-GABARAP antibody (A01907-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HELA whole cell lysates, Lane 5: human U87 whole cell lysates, Lane 6: human HEPG2 whole cell lysates, Lane 7: human U937 whole cell lysates, Lane 8: rat liver tissue lysates, Lane 9: rat pancreas tissue lysates, Lane 10: mouse liver tissue lysates, Lane 11: mouse pancreas tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GABARAP antigen affinity purified polyclonal antibody (Catalog # A01907-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GABARAP at approximately 18KD. The expected band size for GABARAP is at 18KD.
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