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ProductsBack/GABARAP (E-8) Alexa Fluor(r) 790

GABARAP (E-8) Alexa Fluor(r) 790

SC-377300AF790
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGABARAP
200 ug
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    GABARAP (E-8) Alexa Fluor(r) 790
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Conjugate
    AlexaFluor 790
  • Gene ID11337
  • Target name
    GABARAP
  • Target description
    GABA type A receptor-associated protein
  • Target synonyms
    ATG8A, GABARAP-a, MM46, gamma-aminobutyric acid receptor-associated protein, GABA(A) receptor-associated protein, epididymis secretory sperm binding protein
  • Host
    Mouse
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GABARAP using anti-GABARAP antibody (A01907-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HELA whole cell lysates, Lane 5: human U87 whole cell lysates, Lane 6: human HEPG2 whole cell lysates, Lane 7: human U937 whole cell lysates, Lane 8: rat liver tissue lysates, Lane 9: rat pancreas tissue lysates, Lane 10: mouse liver tissue lysates, Lane 11: mouse pancreas tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GABARAP antigen affinity purified polyclonal antibody (Catalog # A01907-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GABARAP at approximately 18KD. The expected band size for GABARAP is at 18KD.
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