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Goat anti-GABRB3 Antibody

ORB20106
Biorbyt
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityBovine, Canine, Human, Mouse, Rat
TargetGABRB3
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Goat anti-GABRB3 Antibody
  • Delivery Days Customer
    16
  • Application Supplier Note
    ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.IHC: In paraffin embedded Human Cortex shows patchy staining near or at the neuronal cell membrane.. Recommended concentration, 2-4ug/ml.WB: Preliminary experiments in Human Brain (Cerebral Cortex, Frontal Cortex and Cerebellum) lysates gave no specific signal but low background (at antibody concentration up to 1 microg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA: 1:8000, WB: 1 μg/ml, IHC-P: 2-4µg/ml ELISA, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2562
  • Target name
    GABRB3
  • Target description
    gamma-aminobutyric acid type A receptor subunit beta3
  • Target synonyms
    DEE43, ECA5, EIEE43, gamma-aminobutyric acid receptor subunit beta-3, GABA-alpha receptor beta-2 subunit, GABAA receptor beta-3 subunit, GABAAR subunit beta-3, gamma-aminobutyric acid (GABA) A receptor, beta 3, gamma-aminobutyric acid A receptor beta 3, gamma-aminobutyric acid type A receptor beta3 subunit, gamma-aminobutyric acid type A receptor subunit beta 3
  • Host
    Goat
  • Scientific Description
    Goat polyclonal antibody to GABRB3
  • Reactivity
    Bovine, Canine, Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GABRB3 using anti-GABRB3 antibody (PB9595). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Brain Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GABRB3 antigen affinity purified polyclonal antibody (Catalog # PB9595) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GABRB3 at approximately 54 kDa. The expected band size for GABRB3 is at 54 kDa.
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